Molecular typing of foodborne coagulase-positive Staphylococcus isolates identified by MALDI-TOF MS
نویسندگان
چکیده
منابع مشابه
Potential of MALDI-TOF MS as an alternative approach for capsular typing Streptococcus pneumoniae isolates
Streptococcus pneumoniae can be classified in more than 90 capsular types, as traditionally determined by serological methods and more recently by PCR-based techniques. Such methods, however, can be expensive, laborious or unable to accurately discriminate among certain serotypes. Therefore, determination of capsular types, although extremely important for epidemiological purposes and for estim...
متن کاملUsing MALDI-TOF-MS to test Staphylococcus aureus–infected vitreous
PURPOSE This study aimed to establish a method for testing Staphylococcus aureus in the vitreous of endophthalmitis with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), which is simple, fast, and sensitive. METHODS S. aureus at different numbers was either mixed with homogenized vitreous or inoculated in porcine eyes for culturing, followed by homo...
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We read with interest the recent report by Ulger Toprak et al. [1] reporting the first case of Butyricimonas virosa bacteraemia published earlier this year. Here we describe the case of a 72year-old man with colon cancer who presented with septic shock after aortic aneurysm surgery, with Butyricimonas virosa recovered from blood cultures. This anaerobic bacteria and related species had been pre...
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Matrix-assisted laser desorption (MALDI) time-offlight (TOF) mass spectrometry (MS) has been successfully used for oligonucleotide sequence confirmation by mass analysis of fragments generated by concentration-dependent exonuclease digestion. This approach is particularly well-suited to the analysis of synthetic DNA due to the relatively short length of the DNA strands. Additionally, an importa...
متن کاملHigh Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile
Clostridium difficile strains were typed by a newly developed MALDI-TOF method, high molecular weight typing, and compared to PCR ribotyping. Among 500 isolates representing 59 PCR ribotypes a total of 35 high molecular weight types could be resolved. Although less discriminatory than PCR ribotyping, the method is extremely fast and simple, and supports for cost-effective screening of isolates ...
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ژورنال
عنوان ژورنال: Acta Alimentaria
سال: 2020
ISSN: 0139-3006,1588-2535
DOI: 10.1556/066.2020.49.3.9